IPTC 2019 - 12th International Particle Toxicology Conference

11-13 September 2019,
Salzburg, Austria
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In vitro – in vivo extrapolation workshop

REGISTER via https://form.jotformeu.com/92123061016341

Aim of Workshop

The PATROLS project is on developing in vitro models that can be used to predict the effect of long term exposure to nanomaterials in humans. In order to get any of the in vitro models accepted for risk assessment, it has to be demonstrated that the predictive value is close to, equal or superior compared to any in vivo study. How can this be done?

The aim of the workshop is to present the quantitative methods to estimate the dose, in vivo and in vitro, in order to explain better the dose-response of engineered nanomaterials (ENM) and to enable the comparison of in vitro and in vivo results. The workshop will be driven by the users’ needs for extrapolation and their options to provide input in the modelling.  

In order to translate the in vitro model outputs to an in vivo or human health outcome, input in the modelling is needed: so what data will that be and what can be provided?  What additional support is needed from the AOPs to get from exposure to adverse health outcome? What can cause lung fibrosis? What are good indicators that can be used in in vitro models: inflammation, cell proliferation, or genotoxicity?   What kind of data and how can it be used for modelling? How much is needed to cause the effect in vivo (rodents) or humans? How does translates back to repeated exposures in vitro? Do the modellers have all critical data for such a backward calculation? What data is still missing to get a good idea on clearance rates and organ burdens?

All these questions will be addressed in this interactive workshop at which active participation of all delegates is needed.

The workshop consists of a series of introductory presentations to set the scene and a hand-on demonstration where the IVIVE extrapolation will be demonstrated using a relatively strong ENM dataset (TiO2) and data currently generated by other WP of PATROLS. Each presenter will reflect on the question raised above as well as those phrased by the participants.

Methods to extrapolate the in vitro results to their in vivo equivalents will be demonstrated. This approach will incorporate the dose estimation in vitro) and the in vivo dose equivalent (where such doses are not available through direct measurements, a Physiologically-Based-Pharmaco-Kinetics (PBPK) model  will be used to estimate the ENM). The scaling from in vitro to in vivo will make use of various scaling factors reflecting the uncertainty in inter-animal and in vitro and in vivo uncertainties.

 The workshop will finish with an IVIVE demonstration on the case-study on lung fibrosis following inhalation of TiO2 will be an interactive session where the audience can participate.

 

Friday, 13 September 2019

(right after the conference) Room: HS 421

13:00

13:30

Registration

13:30

13:45

Introduction, how to get from in vitro to in vivo vice versa

13:45

14:15

What are the main findings from in vivo studies: exposure characterization and adverse effects

14:15

14:45

How do we get from in vitro to in vivo in terms of effects? Are AOPs the tool?

14:45

15:15

What data can in vitro models (on lung fibrosis) provide?

15:15

15:30

BREAK

15:30

16:00

What information is needed to do IVIVE: QSAR? PBPK modelling?

16:00

16:30

From in vivo exposure to in vitro exposure

16:30

17:00

From in vitro effect

17:00

17:30

General discussion and formulating outcomes

17:30

18:00

Adjourn

Contact

 

DECHEMA e.V.
Theodor-Heuss-Allee 25
60486 Frankfurt am Main

Matthias Neumann
Phone: +49 (0)69 7564-254
Email:

 

DECHEMA e.V.

 

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